6C8L

RNA-activated 2-AIpG monomer complex, 1h soaking

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	99
Detector technology	CCD
Collection date	2017-10-25
Detector	MAR CCD 130 mm
Wavelength(s)	1
Spacegroup name	P 3 2 1
Unit cell lengths	47.948, 47.948, 82.388
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.250
R-factor	0.17389
Rwork	0.170
R-free	0.25270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dhc
RMSD bond length	0.025
RMSD bond angle	2.984
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.068	0.426
Rmeas	0.073	0.454
Rpim	0.023	0.154
Number of reflections	5038	255
<I/σ(I)>	34.67	2.33
Completeness [%]	90.6	47.7
Redundancy	9.5	7.8
CC(1/2)	0.990	0.957

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	10% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.040 M Sodium cacodylate trihydrate pH 7.0, 0.012 M Spermine tetrahydrochloride, 0.080 M Sodium chloride, 0.020 M Magnesium chloride