6C8K
RNA-activated 2-AIpG monomer complex, 30 min soaking
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 99 |
| Detector technology | CCD |
| Collection date | 2017-10-25 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 48.103, 48.103, 82.197 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.259 |
| R-factor | 0.22735 |
| Rwork | 0.223 |
| R-free | 0.30223 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dhc |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.624 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.091 | 0.354 |
| Rmeas | 0.101 | 0.396 |
| Rpim | 0.042 | 0.175 |
| Number of reflections | 4919 | 508 |
| <I/σ(I)> | 22 | 2.22 |
| Completeness [%] | 92.3 | 55.2 |
| Redundancy | 5.3 | 4.3 |
| CC(1/2) | 0.988 | 0.954 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 10% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.040 M Sodium cacodylate trihydrate pH 7.0, 0.012 M Spermine tetrahydrochloride, 0.080 M Sodium chloride, 0.020 M Magnesium chloride |
