6C8J

RNA-activated 2-AIpG monomer complex, 15 min soaking

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	99
Detector technology	CCD
Collection date	2017-07-09
Detector	MAR CCD 130 mm
Wavelength(s)	1
Spacegroup name	P 3 2 1
Unit cell lengths	49.091, 49.091, 81.097
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 1.500
R-factor	0.21261
Rwork	0.211
R-free	0.24415
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dhc
RMSD bond length	0.030
RMSD bond angle	3.237
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.110	0.553
Rmeas	0.116	0.586
Rpim	0.036	0.189
Number of reflections	16980	1761
<I/σ(I)>	12.41	3.76
Completeness [%]	90.1	95.7
Redundancy	10.6	9.1
CC(1/2)	0.981	0.918

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	10% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.040 M Sodium cacodylate trihydrate pH 7.0, 0.012 M Spermine tetrahydrochloride, 0.080 M Sodium chloride, 0.020 M Magnesium chloride hexahydrate