6C8G

Crystal structure of Transient Receptor Potential (TRP) channel TRPV4 in the presence of barium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-15
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.7106
Spacegroup name	P 4 21 2
Unit cell lengths	165.519, 165.519, 102.691
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 6.310
R-factor	0.36922
Rwork	0.370
R-free	0.36524
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bbj
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0124)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	6.520
High resolution limit [Å]	6.300	6.300
Rpim	0.030	0.427
Number of reflections	3348	320
<I/σ(I)>	30.7
Completeness [%]	99.8
Redundancy	9.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	50 mM MES pH6.0, 100 mM NaCl, 5.5% PEG4000, and 10% glycerol