6C68
MHC-independent t cell receptor A11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-18 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 89.240, 102.555, 118.503 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.020 - 2.590 |
| R-factor | 0.2046 |
| Rwork | 0.202 |
| R-free | 0.25810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3c5z |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.290 |
| Data reduction software | SCALEIT |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.650 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.095 | 0.487 |
| Number of reflections | 32187 | 1262 |
| <I/σ(I)> | 23 | |
| Completeness [%] | 94.7 | 68.4 |
| Redundancy | 9.9 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG 4000, 0.1M Tris, pH8.5, 10mM MgCl2 |
