6C64
Crystal Structure of the Mango-II Fluorescent Aptamer Bound to TO3-Biotin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 37.098, 181.764, 108.289 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.515 - 3.000 |
| R-factor | 0.196349464513 |
| Rwork | 0.190 |
| R-free | 0.25508 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6c63 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.520 | 3.190 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.098 | 0.930 |
| Number of reflections | 7735 | 1268 |
| <I/σ(I)> | 4.3 | 0.5 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 10.5 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 0.1 M HEPES pH 7.5, 2.45 M Ammonium Formate, 12.5% glycerol, 3% sorbitol |
