6C5R
Crystal structure of the soluble domain of the mitochondrial calcium uniporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-25 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 261.785, 119.921, 88.005 |
| Unit cell angles | 90.00, 106.89, 90.00 |
Refinement procedure
| Resolution | 42.105 - 3.096 |
| R-factor | 0.263784373481 |
| Rwork | 0.262 |
| R-free | 0.30640 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.660 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | phenix.refine (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.170 |
| High resolution limit [Å] | 3.096 | 3.100 |
| Rmerge | 0.089 | |
| Number of reflections | 47337 | 3153 |
| <I/σ(I)> | 14.1 | |
| Completeness [%] | 100.0 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M glycine, pH 9.3, 15.5% PEG 4000, and 0.6 M NaNO3 |
