6C59
Chimeric Pol kappa RIR Rev1 C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-31 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 47.220, 47.220, 89.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.390 - 2.030 |
| R-factor | 0.1862 |
| Rwork | 0.184 |
| R-free | 0.21870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fjo |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.729 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.773 | 2.080 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmeas | 0.118 | 0.873 |
| Number of reflections | 7068 | 503 |
| <I/σ(I)> | 20.8 | 4.34 |
| Completeness [%] | 99.9 | |
| Redundancy | 15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | HEPES pH 7.2, KCl, CHAPS, TCEP, sodium acetate, PEG 4000, isopropanol |
