6C52

Cross-alpha Amyloid-like Structure alphaTet

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.11584
Spacegroup name	P 21 21 2
Unit cell lengths	47.450, 75.570, 27.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.185 - 1.600
R-factor	0.1935
Rwork	0.192
R-free	0.22250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.887
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.000	1.650
High resolution limit [Å]	1.600	1.600
Number of reflections	25538	2532
<I/σ(I)>	21.3	3.3
Completeness [%]	98.9	97.6
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	2.0 M Na formate, 0.1 M Na acetate