6C3R
Cricket paralysis virus RNAi suppressor protein CrPV-1A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-12-17 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | .97943 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.854, 52.117, 111.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.600 |
| R-factor | 0.2218 |
| Rwork | 0.219 |
| R-free | 0.27980 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.319 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 7.030 | 2.600 |
| Rmerge | 0.082 | 0.025 | 0.917 |
| Number of reflections | 9872 | 569 | 479 |
| <I/σ(I)> | 10.9 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 4.7 | 4.2 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 296 | PEG 3350 Lithium chloride |
