6C3B
O2-, PLP-Dependent L-Arginine Hydroxylase RohP Holoenzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-01-28 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97949 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 79.380, 112.410, 102.160 |
Unit cell angles | 90.00, 95.77, 90.00 |
Refinement procedure
Resolution | 56.686 - 1.510 |
R-factor | 0.1533 |
Rwork | 0.152 |
R-free | 0.17250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5dj1 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.873 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless (0.5.23) |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 56.690 | 56.690 | 1.540 |
High resolution limit [Å] | 1.510 | 8.270 | 1.510 |
Rmerge | 0.079 | 0.036 | 0.779 |
Rmeas | 0.090 | 0.042 | 0.898 |
Rpim | 0.044 | 0.021 | 0.440 |
Number of reflections | 139369 | 6931 | |
<I/σ(I)> | 9.7 | ||
Completeness [%] | 100.0 | 98.5 | 99.9 |
Redundancy | 4.2 | 4 | 4 |
CC(1/2) | 0.997 | 0.996 | 0.660 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.2 M sodium malonate pH 7, 16-20% (w/v) PEG 3350 |