6C37
Mycobacterium smegmatis RimJ in complex with CoA-disulfide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-16 |
| Detector | RAYONIX MX325HE |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 64.131, 64.131, 230.906 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.761 - 1.696 |
| R-factor | 0.1933 |
| Rwork | 0.192 |
| R-free | 0.21220 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.006 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 |
| High resolution limit [Å] | 1.696 | 1.696 |
| Rmerge | 0.961 | |
| Number of reflections | 53962 | |
| <I/σ(I)> | 19.7 | |
| Completeness [%] | 91.6 | |
| Redundancy | 11.5 | |
| CC(1/2) | 0.755 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 mM sodium chloride, 0.1 M sodium potassium phosphate (pH 6.2) and 10 % w/v polyethylene glycol 8000 |
