6C34

Mycobacterium smegmatis DNA flap endonuclease mutant D125N

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	63.246, 39.878, 68.424
Unit cell angles	90.00, 108.80, 90.00

Refinement procedure

Resolution	50.000 - 2.200
R-factor	0.1809
Rwork	0.177
R-free	0.22640
RMSD bond length	0.007
RMSD bond angle	0.938
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.250
High resolution limit [Å]	2.200	2.200
Rpim	0.075	0.420
Number of reflections	31716
<I/σ(I)>	5.9	1.4
Completeness [%]	99.0	99.9
Redundancy	4.1	4.1
CC(1/2)	0.995	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2 M NaCl, 20% PEG3350