6C30
Mycobacterium smegmatis RimJ (apo form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-15 |
| Detector | RAYONIX MX325HE |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.414, 79.408, 116.156 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.207 - 1.397 |
| R-factor | 0.1743 |
| Rwork | 0.174 |
| R-free | 0.19510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.129 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.450 |
| High resolution limit [Å] | 1.397 | 1.400 |
| Rmerge | 0.082 | 0.867 |
| Number of reflections | 748457 | 103579 |
| <I/σ(I)> | 25.3 | 2.4 |
| Completeness [%] | 99.7 | 97.8 |
| Redundancy | 7.2 | |
| CC(1/2) | 0.772 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 24 % w/v polyethylene glycol 1500, 20 % v/v glycerol |
