6BZU
Structure of the Hepatitis C virus envelope glycoprotein E2 antigenic region 412-423 bound to the broadly neutralizing antibody 19B3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-15 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 132.696, 55.600, 155.259 |
| Unit cell angles | 90.00, 106.20, 90.00 |
Refinement procedure
| Resolution | 29.819 - 2.700 |
| R-factor | 0.2663 |
| Rwork | 0.265 |
| R-free | 0.28990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4g6a |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.586 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.820 | 2.770 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 59853 | |
| <I/σ(I)> | 8.5 | |
| Completeness [%] | 98.6 | |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% (w/v) PEG 3500, 0.2 M sodium-thicoyanate, pH 6.9 |
