6BZS

Human ABCC6 NBD1 in Apo state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-11-30
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.541780
Spacegroup name	P 32 2 1
Unit cell lengths	94.204, 94.204, 46.110
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.792 - 2.300
R-factor	0.215022917727
Rwork	0.210
R-free	0.26355
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cbz
RMSD bond length	0.004
RMSD bond angle	0.656
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.790	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.083	0.299
Rmeas	0.091	0.328
Number of reflections	10717	1043
<I/σ(I)>	6	2.2
Completeness [%]	100.0	100
Redundancy	5.94	5.94

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	2.1-2.4M Ammonium Sulfate 0.1M HEPES pH 6.8-8.2