6BZR

Human ABCC6 NBD2 in ADP-bound state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2017-02-07
Detector	RIGAKU SATURN 944
Wavelength(s)	1.541780
Spacegroup name	P 1 21 1
Unit cell lengths	55.760, 93.120, 64.170
Unit cell angles	90.00, 112.50, 90.00

Refinement procedure

Resolution	49.342 - 2.800
R-factor	0.234182414144
Rwork	0.231
R-free	0.26407
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gd7
RMSD bond length	0.014
RMSD bond angle	1.234
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.342	2.870
High resolution limit [Å]	2.800	2.800
Number of reflections	29279	2127
<I/σ(I)>	7.9	1.93
Completeness [%]	99.4	100
Redundancy	4.36
CC(1/2)	0.934	0.320

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	277.15	0.1 M Sodium Citrate pH 6.2, 12.5% PEG 4000, 18% propanol