6BZQ
Crystal structure of halogenase PltM in complex with FAD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-16 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.304, 157.711, 213.545 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.750 |
| R-factor | 0.23111 |
| Rwork | 0.230 |
| R-free | 0.26133 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6bzn |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.095 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.810 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.150 | 0.820 |
| Number of reflections | 54483 | 3369 |
| <I/σ(I)> | 12 | |
| Completeness [%] | 96.0 | 97.5 |
| Redundancy | 4.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | 0.1 M Tris pH 8, 0.2 M NaBr, 0.1 M CaCl2 and 14% PEG 8000, frozen in same with the exception of using 16% PEG 8000 and 1 mM FAD |
