6BZH

Structure of mouse RIG-I tandem CARDs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.95371
Spacegroup name	P 21 21 21
Unit cell lengths	90.190, 97.560, 113.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.926 - 2.500
R-factor	0.2289
Rwork	0.226
R-free	0.27510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4a2q
RMSD bond length	0.002
RMSD bond angle	0.501
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.926	2.589
High resolution limit [Å]	2.500	2.500
Rmerge	0.182
Rmeas		1.379
Number of reflections	35016
<I/σ(I)>	8.73	1.84
Completeness [%]	99.0
Redundancy	4.3
CC(1/2)	0.988	0.466

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		281	0.2 M ammonium sulphate 26% w/v polyethylene glycol 3350 0.1 M Bis-Tris, pH 6.5.