6BZD

Structure of 14-3-3 gamma R57E mutant bound to GlcNAcylated peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	76.366, 60.000, 125.786
Unit cell angles	90.00, 90.50, 90.00

Refinement procedure

Resolution	65.024 - 2.670
R-factor	0.2469
Rwork	0.246
R-free	0.28120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5USK
RMSD bond length	0.005
RMSD bond angle	0.656
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.024	2.800
High resolution limit [Å]	2.670	2.670
Rpim		0.486
Number of reflections	31410
<I/σ(I)>	14.3	5.8
Completeness [%]	95.7
Redundancy	2.5
CC(1/2)	0.997	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	25% PEG 4000, 0.2 M MgCl2, 0.1 M Tris pH 8.5, 20% glycerol