6BZC

Crystal Structure of Glucose-6-phosphate Isomerase from Elizabethkingia anophelis with bound Glucose-6-phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2017-11-02
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	69.280, 102.140, 153.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.466 - 2.050
R-factor	0.209
Rwork	0.207
R-free	0.27640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bzb
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((dev_2919))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.466	48.466	2.100
High resolution limit [Å]	2.050	9.170	2.050
Rmerge	0.103	0.030	0.508
Rmeas	0.119	0.034	0.587
Total number of observations	276270
Number of reflections	68995	869	5027
<I/σ(I)>	9.51	20.46	2.57
Completeness [%]	99.7	97.6	100
Redundancy	4.004	3.582	4.058
CC(1/2)	0.994	0.998	0.726

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	ElanA.17127.a.B1.PS38341 at 26.1 mg/ml was mixed 1:1 with MCSG1(f1): 0.1 M Bis-Tris:HCl, pH=6.5, 20% (w/v) PEG MME 5000. Crystal was then soaked with 5 mM glucose-6-phosphate and 20% ethylene glycol for 4 days. Tray: 295124f1, puck: pqf5-1