6BZ6

Thermus thermophilus 70S complex containing 16S G347U ram mutation and empty A site

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-27
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.972
Spacegroup name	P 21 21 21
Unit cell lengths	209.654, 447.955, 618.805
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	124.481 - 3.180
R-factor	0.2324
Rwork	0.232
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v97
RMSD bond length	0.009
RMSD bond angle	0.990
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.12)
Refinement software	PHENIX (1.12)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	124.481	124.481	3.260
High resolution limit [Å]	3.180	14.220	3.180
Rmerge	0.304	0.043	2.150
Rmeas	0.326	0.046	2.313
Total number of observations	7443829
Number of reflections	958766	11129	70464
<I/σ(I)>	7.97	37.4	1.03
Completeness [%]	99.3	96.5	99.3
Redundancy	7.764	7.298	7.464
CC(1/2)	0.995	0.998	0.240

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	4.8% PEG 20k, 4.8% PEG 550MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC