6BYW
Structure of GoxA from Pseudoalteromonas luteoviolacea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 109.843, 93.236, 188.457 |
| Unit cell angles | 90.00, 94.88, 90.00 |
Refinement procedure
| Resolution | 48.283 - 2.050 |
| R-factor | 0.1872 |
| Rwork | 0.185 |
| R-free | 0.21920 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.780 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.283 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.094 | 0.603 |
| Rmeas | 0.111 | 0.720 |
| Rpim | 0.058 | 0.522 |
| Number of reflections | 235829 | 11390 |
| <I/σ(I)> | 10.9 | 2.7 |
| Completeness [%] | 99.3 | 97.5 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7.5 | 292 | Batch mode under paraffin oil. 1:1 mixture of protein at 10 mg/mL with mother liquor containing 0.1 M HEPES pH 7.5, 0.1 M ammonium sulfate, 20% PEG 3350 |
