6BYM

Crystal structure of the sterol-bound second StART domain of yeast Lam4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2017-11-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00001
Spacegroup name	P 1
Unit cell lengths	38.179, 47.301, 55.996
Unit cell angles	111.21, 101.63, 100.33

Refinement procedure

Resolution	42.381 - 2.200
R-factor	0.1688
Rwork	0.166
R-free	0.22960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.999
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.280
High resolution limit [Å]	2.200	4.740	2.200
Rmerge	0.090	0.060	0.259
Rmeas	0.105	0.070	0.301
Rpim	0.053	0.035	0.153
Total number of observations	66375
Number of reflections	17147	1733	1682
<I/σ(I)>	9.6
Completeness [%]	98.3	99.5	97.1
Redundancy	3.9	3.9	3.8
CC(1/2)		0.995	0.952

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293.15	Lithium Chloride 0.7M 0.1M HEPES pH 7.5 24% PEG6000