6BYL

Structure of 14-3-3 gamma bound to O-GlcNAcylated thr peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-03-26
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	121.200, 121.200, 310.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	112.923 - 3.350
R-factor	0.237
Rwork	0.234
R-free	0.28480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5USK
RMSD bond length	0.003
RMSD bond angle	0.466
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	112.923	3.530
High resolution limit [Å]	3.350	3.350
Rmeas	0.115	1.003
Rpim	0.068	0.613
Number of reflections	32119	4440
<I/σ(I)>	6.2	1.2
Completeness [%]	94.5	91.5
Redundancy	2.3	2.2
CC(1/2)	0.997	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	29% PEG 4000, 200 mM sodium acetate, 0.1 M Tris pH 8.5