6BYD

Crystal structure of the second StART domain of yeast Lam4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2017-09-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97494
Spacegroup name	I 21 21 21
Unit cell lengths	45.601, 67.923, 132.042
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.103 - 2.195
R-factor	0.189
Rwork	0.186
R-free	0.24800
RMSD bond length	0.008
RMSD bond angle	0.901
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL2Map
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.270
High resolution limit [Å]	2.190	4.720	2.190
Rmerge	0.132	0.078	0.664
Rmeas	0.137	0.081	0.691
Rpim	0.037	0.022	0.189
Total number of observations	153681
Number of reflections	11009	1196	1083
<I/σ(I)>	7.3
Completeness [%]	100.0	100	100
Redundancy	14	13.3	12.8
CC(1/2)		0.997	0.968

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293.15	0.5M Lithium Chloride 30% PEG 6000 0.1M HEPES, pH 7.5