6BY3
Open and conductive conformation of KcsA-T75A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-23 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.92 |
| Spacegroup name | I 4 |
| Unit cell lengths | 156.126, 156.126, 74.479 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.799 - 2.370 |
| R-factor | 0.2057 |
| Rwork | 0.205 |
| R-free | 0.21880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.572 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 6.370 | 2.350 |
| Rmerge | 0.153 | 0.104 | 0.447 |
| Total number of observations | 141801 | ||
| Number of reflections | 38119 | 1915 | 1715 |
| <I/σ(I)> | 13.8 | ||
| Completeness [%] | 98.6 | 95.1 | 91.1 |
| Redundancy | 3.7 | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG400, 50 mM magnesium acetate, 50 mM sodium acetate |
