6BY2
Closed and deep-inactivated conformation of KcsA-T75A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL14-1 |
Synchrotron site | SSRL |
Beamline | BL14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-05-23 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.2 |
Spacegroup name | I 4 |
Unit cell lengths | 157.178, 157.178, 75.459 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.825 - 2.350 |
R-factor | 0.2223 |
Rwork | 0.221 |
R-free | 0.24520 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.579 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.430 |
High resolution limit [Å] | 2.350 | 6.370 | 2.350 |
Rmerge | 0.153 | 0.104 | 0.447 |
Number of reflections | 38148 | 1915 | 1715 |
<I/σ(I)> | 7.67 | ||
Completeness [%] | 99.1 | 95.1 | 91.1 |
Redundancy | 3.7 | 3.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG400, 50 mM magnesium acetate, 50 mM sodium acetate |