6BXZ
Crystal Structure of Pig Protocadherin-15 EC10-MAD12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-07 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.284, 49.246, 108.253 |
| Unit cell angles | 90.00, 92.15, 90.00 |
Refinement procedure
| Resolution | 108.180 - 2.090 |
| R-factor | 0.18883 |
| Rwork | 0.187 |
| R-free | 0.21708 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xhz |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.550 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 108.180 | 2.140 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.110 | 0.437 |
| Number of reflections | 57228 | 2452 |
| <I/σ(I)> | 15.2 | 2.45 |
| Completeness [%] | 97.6 | 85.3 |
| Redundancy | 5.3 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1 M HEPES pH 7.5 20 % v/v Jeffamine M-600 |
