6BXX

GYNGFG from low-complexity domain of hnRNPA1, residues 243-248

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	16.606, 4.769, 40.973
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.486 - 1.100
R-factor	0.0638
Rwork	0.061
R-free	0.08650
Structure solution method	AB INITIO PHASING
RMSD bond length	0.018
RMSD bond angle	2.220
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	1.140
High resolution limit [Å]	1.100	2.370	1.100
Rmerge	0.130	0.100	0.248
Total number of observations	32560
Number of reflections	1269	192	41
<I/σ(I)>	8.4
Completeness [%]	78.9	99	26.8
Redundancy	25.7	24.9	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	0.1M sodium cacodylate pH 6.5, 30% (v/v) MPD