6BXU
Crystal Structure of Mouse Protocadherin-15 EC5-7 I582T
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-12-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 42 2 2 |
| Unit cell lengths | 180.905, 180.905, 127.161 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 180.900 - 3.790 |
| R-factor | 0.24169 |
| Rwork | 0.240 |
| R-free | 0.28349 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5w1d |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.486 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 180.900 | 3.870 |
| High resolution limit [Å] | 3.790 | 3.790 |
| Rmerge | 0.231 | |
| Number of reflections | 21629 | 946 |
| <I/σ(I)> | 7.43 | 0.545 |
| Completeness [%] | 97.6 | 87.8 |
| Redundancy | 6.4 | 4.7 |
| CC(1/2) | 0.269 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M Na formate, 40% MPD |
