6BXT
Crystal structure of Toxoplasma gondii Mitochondrial Association Factor 1 A (MAF1A) in complex with ADPribose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.230, 49.520, 115.710 |
| Unit cell angles | 90.00, 96.63, 90.00 |
Refinement procedure
| Resolution | 27.397 - 2.700 |
| R-factor | 0.2352 |
| Rwork | 0.233 |
| R-free | 0.28560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6bxs |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.671 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.400 | 2.860 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.090 | 0.611 |
| Number of reflections | 23134 | |
| <I/σ(I)> | 11.6 | 2.2 |
| Completeness [%] | 94.4 | 87.9 |
| Redundancy | 5.1 | 4.7 |
| CC(1/2) | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.9 M Ammonium sulfate, 0.1 M Hepes pH 7.0, 0.5% PEG8000, 3% 2-methyl-2,4- pentanediol |
