6BXD

Crystal structure of Variable Lymphocyte Receptor 2 (VLR2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.88557
Spacegroup name	P 21 21 2
Unit cell lengths	63.033, 74.731, 40.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.365 - 1.103
R-factor	0.1769
Rwork	0.177
R-free	0.17900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3twi
RMSD bond length	0.004
RMSD bond angle	0.819
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.120
High resolution limit [Å]	1.100	1.100
Rmerge	0.100	0.470
Rpim	0.030	0.370
Number of reflections	74681	2632
<I/σ(I)>	33.4	1.7
Completeness [%]	95.2	68.8
Redundancy	9.5	2.2
CC(1/2)	0.910

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.2 M (NH4)2SO4, 30% PEG 8K