6BWZ

SYSGYS from low-complexity domain of FUS, residues 37-42

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-03
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	17.569, 4.818, 18.283
Unit cell angles	90.00, 91.03, 90.00

Refinement procedure

Resolution	18.280 - 1.100
R-factor	0.1434
Rwork	0.141
R-free	0.16460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3nvh
RMSD bond length	0.014
RMSD bond angle	1.799
Data reduction software	XDS
Data scaling software	SCALEPACK
Phasing software	PHASER (2.5.6)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	1.140
High resolution limit [Å]	1.100	2.370	1.100
Rmerge	0.179	0.114	0.468
Total number of observations	5560
Number of reflections	1182
<I/σ(I)>	6.5
Completeness [%]	82.0	89.5	28.4
Redundancy	4.7	4.4	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	0.1M Bis-Tris pH 6.0, 0.2M Magnesium Formate Dihydrate