6BWN

Crystal Structure of Mouse Protocadherin-15 EC6-7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 62 2 2
Unit cell lengths	110.981, 110.981, 206.024
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	96.110 - 2.940
R-factor	0.21797
Rwork	0.217
R-free	0.24171
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tpk
RMSD bond length	0.008
RMSD bond angle	1.321
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	96.110	3.000
High resolution limit [Å]	2.940	2.950
Rmerge	0.131
Number of reflections	16643	784
<I/σ(I)>	10.9	2
Completeness [%]	98.1	96.9
Redundancy	7.7	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	30% PEG1500