6BWN
Crystal Structure of Mouse Protocadherin-15 EC6-7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 110.981, 110.981, 206.024 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 96.110 - 2.940 |
| R-factor | 0.21797 |
| Rwork | 0.217 |
| R-free | 0.24171 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tpk |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.321 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.110 | 3.000 |
| High resolution limit [Å] | 2.940 | 2.950 |
| Rmerge | 0.131 | |
| Number of reflections | 16643 | 784 |
| <I/σ(I)> | 10.9 | 2 |
| Completeness [%] | 98.1 | 96.9 |
| Redundancy | 7.7 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 30% PEG1500 |
