6BWA

Hendra virus W protein C-terminus in complex with Importin alpha 3 crystal form 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	48.070, 60.040, 89.870
Unit cell angles	90.00, 97.85, 90.00

Refinement procedure

Resolution	19.949 - 2.200
R-factor	0.1879
Rwork	0.186
R-free	0.21550
RMSD bond length	0.003
RMSD bond angle	0.539
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.950	2.270
High resolution limit [Å]	2.200	2.200
Rpim	0.081	0.369
Number of reflections	25833	2232
<I/σ(I)>	9.6	3.5
Completeness [%]	99.6	99.8
Redundancy	3.5	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.2M sodium chloride, 0.1M MES pH6.5, 10% w/v PEG 4000