6BW6

Human GPT (DPAGT1) H129 variant in complex with tunicamycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-31
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9791
Spacegroup name	C 1 2 1
Unit cell lengths	210.615, 105.363, 148.080
Unit cell angles	90.00, 103.49, 90.00

Refinement procedure

Resolution	94.509 - 2.950
R-factor	0.2614
Rwork	0.260
R-free	0.29080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lev
RMSD bond length	0.003
RMSD bond angle	0.576
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	3.055
High resolution limit [Å]	2.950	2.950
Rpim	0.079
Number of reflections	36769	6360
<I/σ(I)>	17.51
Completeness [%]	92.6	54.7
Redundancy	112.4	112.8
CC(1/2)	0.840	0.763

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	PEG400, calcium chloride