6BW0

Nipah virus W protein C-terminus in complex with Importin alpha 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	77.855, 88.818, 97.646
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.273 - 2.100
R-factor	0.1863
Rwork	0.185
R-free	0.20330
RMSD bond length	0.005
RMSD bond angle	0.730
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.610	2.160
High resolution limit [Å]	2.100	2.100
Rpim	0.033	0.253
Number of reflections	38272	3193
<I/σ(I)>	11.7	2.4
Completeness [%]	96.0	97.8
Redundancy	3.6	3.6
CC(1/2)	0.998	0.814

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.01M DTT, 0.1M sodium HEPES pH7, 0.7M sodium citrate