6BVV
Nipah virus W protein C-terminus in complex with Importin alpha 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.701, 65.610, 73.939 |
| Unit cell angles | 90.00, 99.79, 90.00 |
Refinement procedure
| Resolution | 38.211 - 2.300 |
| R-factor | 0.2006 |
| Rwork | 0.199 |
| R-free | 0.22240 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.747 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.211 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rpim | 0.087 | 0.274 |
| Number of reflections | 19881 | 1953 |
| <I/σ(I)> | 8.6 | 3.5 |
| Completeness [%] | 98.9 | 99.2 |
| Redundancy | 2.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M lithium nitrate, 20% w/v PEG3350 |
