6BVK
Ras:SOS:Ras in complex with a small molecule activator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-07-13 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.98 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 184.421, 184.421, 179.008 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.530 - 1.799 |
| R-factor | 0.1564 |
| Rwork | 0.155 |
| R-free | 0.17870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.843 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12rc1_2801: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.530 | 1.863 |
| High resolution limit [Å] | 1.799 | 1.799 |
| Rpim | 0.039 | 0.392 |
| Number of reflections | 141274 | |
| <I/σ(I)> | 18.5 | |
| Completeness [%] | 99.9 | |
| Redundancy | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 292 | 0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0 |
