6BUI
Crystal structure of a membrane protein, crystal form III
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-07-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 140.182, 242.062, 96.235 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 121.310 - 3.270 |
| R-factor | 0.2812 |
| Rwork | 0.280 |
| R-free | 0.30030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6bug |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 121.310 | 3.400 |
| High resolution limit [Å] | 3.270 | 3.300 |
| Number of reflections | 47040 | 4083 |
| <I/σ(I)> | 11.5 | 1.4 |
| Completeness [%] | 97.5 | 98.1 |
| Redundancy | 3.8 | 3.2 |
| CC(1/2) | 0.563 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 100mM HEPES pH7.5, 200mM sodium citrate tribasic dihydrate, 3% (w/v) 1,5-diaminopentane dihydrochloride, 27% (w/v) PEG 400 |
