6BTP
BMP1 complexed with a hydroxamate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2013-11-04 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 90.331, 124.366, 43.642 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.090 - 1.930 |
| R-factor | 0.2041 |
| Rwork | 0.203 |
| R-free | 0.23330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.223 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 124.366 | 10.000 | 2.030 |
| High resolution limit [Å] | 1.927 | 6.090 | 1.930 |
| Rmerge | 0.027 | 0.606 | |
| Rmeas | 0.126 | 0.033 | 0.704 |
| Rpim | 0.046 | 0.013 | 0.256 |
| Total number of observations | 267447 | ||
| Number of reflections | 36873 | 1332 | 5168 |
| <I/σ(I)> | 17.1 | 23.2 | 1.3 |
| Completeness [%] | 97.0 | 98.9 | 94.7 |
| Redundancy | 7.3 | 6.3 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 8-12% PEG 3350 150-200mM KSCN 10mM spermidine |
