6BT5

Human mGlu8 Receptor complexed with L-AP4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-10
Detector	RAYONIX MX225HE
Wavelength(s)	0.9793
Spacegroup name	I 2 2 2
Unit cell lengths	113.650, 166.008, 196.038
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.420 - 2.920
R-factor	0.2536
Rwork	0.253
R-free	0.26720
RMSD bond length	0.005
RMSD bond angle	0.740
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.760	3.070
High resolution limit [Å]	2.920	2.920
Number of reflections	38545
<I/σ(I)>	16.3
Completeness [%]	100.0
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	3.3 M sodium formate, pH 7.0 and 5 mM L-AP4