6BT1

Structure of the human Nocturnin catalytic domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2016-08-16
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.978
Spacegroup name	P 41 21 2
Unit cell lengths	61.900, 61.900, 155.483
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.132 - 1.480
R-factor	0.1529
Rwork	0.151
R-free	0.18650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.030
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.100	1.510
High resolution limit [Å]	1.480	1.480
Number of reflections	51337
<I/σ(I)>	15.781
Completeness [%]	99.8	98
Redundancy	11.6	4.2
CC(1/2)		0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	294	3', 5' Adenosine Diphosphate, Magnesium Chloride, Tris, Sodium Citrate, PEG 4000