6BSY
HIV-1 Rev assembly domain (residues 1-69)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.800, 43.920, 70.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.630 - 2.250 |
| Rwork | 0.228 |
| R-free | 0.25990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lph |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1-2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.630 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.860 | |
| Rmeas | 0.122 | 0.930 |
| Rpim | 0.340 | |
| Number of reflections | 6861 | 675 |
| <I/σ(I)> | 13.73 | 2.61 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 7.1 | 7.25 |
| CC(1/2) | 0.820 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 283.15 | 0.1 M sodium citrate, 25% MPD |
