6BSW

Crystal structure of Xyloglucan Xylosyltransferase 1 ternary form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	123
Detector technology	PIXEL
Collection date	2017-06-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	65.037, 94.889, 145.104
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.428 - 2.156
R-factor	0.1845
Rwork	0.182
R-free	0.22850
RMSD bond length	0.003
RMSD bond angle	0.520
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.430	2.234
High resolution limit [Å]	2.150	2.150
Rmerge	0.114	0.529
Rmeas	0.126	0.126
Rpim	0.052	0.052
Number of reflections	45315	3194
<I/σ(I)>	10.39
Completeness [%]	92.1	65.86
Redundancy	5.5	4.6
CC(1/2)	0.995	0.822

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	Tris-HCl, Na-Nitrate, PEG3350