6BSV

Crystal structure of Xyloglucan Xylosyltransferase binary form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	123
Detector technology	PIXEL
Collection date	2017-06-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	64.929, 94.549, 144.716
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.325 - 2.433
R-factor	0.1863
Rwork	0.184
R-free	0.21630
RMSD bond length	0.003
RMSD bond angle	0.543
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.000	2.520
High resolution limit [Å]	2.433	2.433
Rmerge	0.128	0.489
Rmeas	0.139
Rpim	0.055
Number of reflections	32047	2083
<I/σ(I)>	9.8	2.02
Completeness [%]	93.8	62.44
Redundancy	6.1	4.5
CC(1/2)	0.995	0.827

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	278	Tris, Na-Nitrate, PEG3350, Trehalose