6BSU
Crystal structure of xyloglucan xylosyltransferase I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 123 |
Detector technology | PIXEL |
Collection date | 2017-08-11 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 94.304, 135.683, 113.226 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.528 - 1.497 |
R-factor | 0.1422 |
Rwork | 0.142 |
R-free | 0.16730 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.811 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.530 | 1.551 |
High resolution limit [Å] | 1.497 | 1.497 |
Rmerge | 0.080 | 0.924 |
Rmeas | 0.085 | |
Rpim | 0.028 | 0.525 |
Number of reflections | 112693 | 9911 |
<I/σ(I)> | 14.31 | 1.03 |
Completeness [%] | 96.4 | 83.82 |
Redundancy | 7.8 | 3.7 |
CC(1/2) | 0.998 | 0.381 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | Tris pH 8, Na-Nitrate, PEG3350 |