6BSQ

Enterococcus faecalis Penicillin Binding Protein 4 (PBP4)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	LIQUID ANODE
Source details	BRUKER METALJET
Temperature [K]	100
Detector technology	CMOS
Collection date	2017-07-27
Detector	Bruker PHOTON II
Wavelength(s)	1.34
Spacegroup name	C 1 2 1
Unit cell lengths	124.709, 84.625, 85.093
Unit cell angles	90.00, 109.26, 90.00

Refinement procedure

Resolution	17.512 - 1.800
R-factor	0.1927
Rwork	0.191
R-free	0.21670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PBP5
RMSD bond length	0.015
RMSD bond angle	1.168
Data reduction software	SAINT
Data scaling software	SADABS
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.512	1.830
High resolution limit [Å]	1.800	1.800
Number of reflections	76417
<I/σ(I)>	16.5
Completeness [%]	98.9
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	40 mM potassium phosphate monobasic, 18% PEG800, 22% glycerol