6BSF

Human GR (418-507) in complex with monomeric DNA binding site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-19
Detector	NOIR-1
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	38.345, 87.907, 103.702
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.528 - 2.400
R-factor	0.2075
Rwork	0.206
R-free	0.23340
RMSD bond length	0.014
RMSD bond angle	1.474
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.528	2.486
High resolution limit [Å]	2.400	2.400
Rmerge	0.073	1.354
Rmeas	0.079	1.497
Rpim	0.030	0.628
Number of reflections	14303	1373
<I/σ(I)>	17.8	1.03
Completeness [%]	99.6	99.85
Redundancy	6.7	5.5
CC(1/2)	0.999	0.681

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.05 M MES monohydrate pH 6.0 0.005 M Magnesium sulfate heptahydrate 5% w/v Polyethylene glycol 4,000